Histology Unit

Histology is the study of tissues and organs through the examination of the microscopical architecture of tissues and the relationship between the different types of cells and tissue types found within tissues and organs.

In order to study tissues with a microscope they must be preserved (fixed) and cut into sections thin enough to be translucent. Normally tissue will be fixed in a formalin solution.

Following fixation, blocks of tissue must be cut into thin sections. One way is to make a firm block by dehydration in alcohols and infiltration with paraffin, or some similar agent - a process called embedding. Sections 3 to 10 microns (3 to 10 thousand of a millimeter) in thickness are cut on steel knives mounted in an instrument called a microtome, which has a precise mechanical advance.

The sections are stained with H & E staining and view under light microscope.

In order to preserve the section which has been made from a block of fixed tissues and stained, it is mounted on a glass slide and covered with a thin cover glass by means of a transparent substance which hardens and seals the preparation to make it permanent. Some tissues are stained and then mounted. More often the tissue is placed on the slide first and then stained.

Histology unit of the Faculty of Pharmacy, Universiti Teknologi Mara (UiTM) Puncak Alam Campus have all the facilities to run a histology sample. The faculty welcome to all researcher to use our equipment for research purpose.